A 21-year-old female presents to the physician’s office with complaints of very
painful vulvar ulcers. The patient states she has had the symptoms for approxi-
mately 3 days, and they are worsening. She says that prior to the ulcerations,
there was a burning and tingling sensation of the skin in the same area. She has
noted similar symptoms like this before and has been told it is sexually trans-
mitted. On examination you see multiple extremely tender, vesicular (blister-
like) lesions on an erythematous (red) base on both labia majora of the vulva.
She has a moderate amount of tender inguinal lymph nodes bilaterally. The
physician uses a swab to sample the ulcer and send it off for diagnostic analysis.
What is the most likely diagnosis?
If the test is for deoxyribonucleic acid (DNA), what method would
be used to amplify the fragments of DNA sampled?