CLINICAL CASES
51
therapy (HAART). To overcome drug resistance and find a definitive cure for
HIV infection several efforts are being made to develop vaccines and also use
ribozymes to target HIV mRNA. Because of the molecular understanding of
HIV, the future of anti-HIV therapy seems bright and promises an effective
cure for the AIDS patient.
C O M P R E H E N SIO N Q U E ST IO N S
[5.1]
The current therapeutic strategy for patients who have been infected
with HIV is a multidrug regimen known as highly active antiretroviral
therapy (HAART). One type of drug used in this therapy is a nucleoside/
nucleotide analog, such as didanosine. Which of the following best
describes the mechanism of action of these drugs?
A. They inhibit the synthesis of viral proteins.
B. They directly bind to and inhibit reverse transcriptase.
C. They prevent the hydrolysis of the viral polyprotein.
D. They prematurely terminate the DNA synthesized by reverse
transcriptase.
E. They inhibit the viral enzyme integrase.
[5.2]
Enzyme-linked immunosorbent assay (ELISA) is used for routine
screening of HIV infection and the Western blot test has been success-
fully used as a confirmatory test. Which of the following best describes
the strategy used to confirm the presence of an HIV infection by
Western blot analysis?
GEL ELECTROPHORESIS
BLOT
AND BLOT
INCUBATION
PERFORMED ON
WITH
BLOT DETECTS
A.
HIV protein standards
Patient serum
Presence of anti-HIV
antibodies in serum
B.
Patient serum
Anti-HIV antibodies
Presence of HIV
proteins in serum
C.
Anti-HIV antibody standards
Patient serum
Presence of HIV
proteins in serum
D.
Patient serum
Labeled
Presence of HIV RNA
oligonucleotides
in serum
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